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Vol 11, No 2 (2025)

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Original study articles

Histomorphological analysis in panel forensic medical examinations in stillbirth cases

Berlay M.V., Kildyushov E.M., Fedko I.I., Avanesyan H.A., Borschevskaya V.N., Zolotukhina E.A., Karpov S.M.

Abstract

BACKGROUND: Panel forensic medical examinations are among the methods used by Russian investigative authorities for preliminary inquiries and criminal investigations into inadequate obstetric care. Forensic histological analysis of archival autopsy materials and placental tissue is a critical component of panel forensic medical examinations when investigating intrauterine fetal death.

AIM: The work aimed to analyze panel forensic medical examinations in stillbirth cases and determine the need for forensic histological examination of archival autopsy materials and placental tissue based on current scientific approaches.

METHODS: An observational, single-center, cross-sectional study was conducted using archival records from the Bureau of Forensic Medical Examination of Stavropol Territory. Inclusion criteria: cases of panel forensic medical examinations related to intrauterine fetal death (both antenatal and intranatal).

RESULTS: The study included panel forensic medical examinations of intrauterine fetal death cases (n = 68) from January 1, 2015, to December 31, 2022. In 79.4% of cases (n = 54), the examinations were initiated by investigators from the Investigative Committee; in 16.2% (n = 11), by investigators/officers from the Ministry of Internal Affairs; and in 4.4% (n = 3), by judges. In 86.7% of cases, the examinations were conducted as part of preliminary inquiries. The causes of stillbirth were antenatal fetal death in 57.4% (n = 39), intranatal fetal death in 39.7% (n = 27), and congenital abnormalities in 2.9% (n = 2). In 97.1% (n = 66) of panel examinations involving intrauterine fetal death, histologists performed forensic histological examination of autopsy material and the placenta.

CONCLUSION: The analysis showed that 39.1% of panel forensic medical examinations in obstetrics and gynecology were related to stillbirth. To improve the quality of expert examinations in this area, forensic histological examinations of autopsy material and the placenta are required. Improving the methodology for panel forensic medical examinations in stillbirth is a pressing issue that necessitates interdisciplinary collaboration among forensic medical experts, pathologists, obstetricians-gynecologists, and neonatologists.

Russian Journal of Forensic Medicine. 2025;11(2):112-122
pages 112-122 views

A pilot study on forensic odontologists’ practices in the registration and analysis of dog bite marks in humans

Angelakopoulos N., Polukhin N.V., Balla S.B., Zolotenkova G.V.

Abstract

BACKGROUND: Dog bite marks and injuries on humans is a significant public health problem with a relatively major impact on economic productivity and quality of life.

AIM: To investigate current practices and perspectives of forensic odontologists regarding the analysis of dog bite marks, focusing on key aspects such as documentation protocols, canine examinations, DNA collection, and legal considerations across various jurisdictions.

METHODS: A survey was conducted among 22 forensic odontologists to obtain insights into their experiences and practices related to dog bite mark investigations. The respondents were asked about their approaches to documenting bite marks, examining suspect dogs, collecting biological evidence, and complying with legal requirements in different countries.

RESULTS: The sample population comprised an equal number of male and female participants, with a mean age of 47.3 ± 13.6 years. The majority (63.6%) were certified forensic odontologists, while 9.1% were pursuing advanced degrees. More than half (54.5%) had over 15 years of experience in the field. Approximately one-third (36.4%) reported having encountered dog bite cases. The majority of the respondents (95.4%) recognized the importance of photographing bite marks, with significant differences in opinion observed based on career stage (p = 0.008). Furthermore, respondents commonly utilized alginate, silicone, or other soft-body materials (50%) or digital photogrammetric scanning (36.4%) to obtain bite mark impressions. Despite the relatively limited adoption of photogrammetric scanning, 68.2% of forensic odontologists recommended its use. Additionally, 72.8% of respondents supported the necessity of collecting DNA and bacterial swabs. A substantial proportion (77.3%) acknowledged a lack of familiarity with specific dog profiling kits. Moreover, the majority (68.2%) endorsed the implementation of a standardized checklist to ensure accurate and consistent documentation of dog bite marks.

CONCLUSIONS: The survey findings highlight consensus on fundamental investigative steps including photographing bite marks and conducting canine examinations. However, the practices related to DNA collection and legal requirements across jurisdictions vary. Standardized protocols and further research are required to enhance the reliability and effectiveness of forensic analyses in this critical domain.

Russian Journal of Forensic Medicine. 2025;11(2):123-136
pages 123-136 views

Technical reports

Selecting thermometer accuracy in estimating the postmortem interval using body temperature-based methods: A technical report

Vavilov A.Y., Khalikov A.A., Naidenova T.V.

Abstract

BACKGROUND: Establishing the postmortem interval of a deceased individual with the highest possible accuracy is critical for the objective investigation of criminal homicide. It is well known that instrumental error in measuring a parameter used in calculations is one of the primary sources of error in any computational method. However, in body temperature-based methods for estimating postmortem interval, the accuracy of temperature measurement adequate for forensic medicine has not been established.

AIM: The work aimed to develop practical recommendations for selecting a measuring instrument for postmortem examination based on the impact of body temperature measurement accuracy and environmental factors on the error in calculated postmortem interval.

METHODS: Using the phenomenological mathematical model by Henssge et al., changes in postmortem body temperature were predicted in various diagnostic zones (brain, liver, rectum) at ambient temperatures of 0 °C and 20 °C. The method’s instrumental error was calculated for these conditions using thermometers with accuracies of 1 °C, 0.1 °C, and 0.01 °C. The results were expressed as both absolute and relative errors (%), which were calculated as the ratio of error to the postmortem interval at which it was obtained.

RESULTS: The highest relative errors were reported during the early phase of postmortem cooling and during the stage of temperature equalization between the body and the environment. In all cases, using thermometers with an accuracy of 1 °C resulted in a relative error of more than 15% of the postmortem interval value. Moreover, the use of thermometers with an accuracy of 0.1 °C or 0.01 °C ensured a relative instrumental error of no more than 5% throughout the modeling period (2–24 hours).

CONCLUSION: According to biomedical research guidelines, satisfactory results can only be achieved when body and ambient temperatures are measured with an accuracy of 0.1 °C or 0.01 °C. For practical convenience, we implemented the algorithm for calculating the instrumental error in postmortem interval estimation in the form of a Microsoft Excel spreadsheet.

Russian Journal of Forensic Medicine. 2025;11(2):137-144
pages 137-144 views

Online histostereometric analysis in digital forensic pathology: a technical report

Nedugiv V.G., Zhukova A.V., Nedugov G.V.

Abstract

BACKGROUND: Quantitative image analysis of histological, histochemical, and immunohistochemical specimens is an essential component of digital forensic pathology. However, the scarcity of commercial analysis software limits the widespread implementation of digital pathology principles, and thus objective histological diagnosis, in forensic medical examinations in Russia. This article presents a readily accessible online application for automated histostereometric image analysis of histological and immunohistochemical specimens, as well as digital photographs of individual fields of view.

AIM: The work aimed to develop an online tool for histostereometric analysis of images used in digital forensic pathology.

METHODS: This work presents an online application compatible with Windows, Linux, Android, and iOS operating systems. The application is designed to detect microstructures with specific color characteristics in digital images and perform histostereometric analysis. The software code was written in JavaScript using the open-source library OpenCV.

RESULTS: An online application Color Histostereometry Calculator was developed to determine the relative volume and number of microstructures with specific color characteristics in raster images of histological and immunohistochemical specimens. The application uses the HSV (Hue, Saturation, Value) color model, with the ability to adjust the ranges of color parameters and the minimum size of the analyzed regions; moreover, it identifies microstructures based on their color characteristics rather than geometric features. This allows for the exclusion of various image artifacts from the analysis, the segmentation of overlapping structures, and the evaluation of morphometric parameters for an infinitesimally thin section, thereby eliminating the influence of section thickness on the analysis results.

CONCLUSION: The proposed online application is recommended for histostereometric analysis in digital forensic pathology.

Russian Journal of Forensic Medicine. 2025;11(2):145-154
pages 145-154 views

Reviews

Expert methods for determining the speed of a vehicle at the moment of collision with a pedestrian: current state of the issue (a review)

Vavilov A.Y., Vasilev D.N., Timerzyanov M.I.

Abstract

Knowing the vehicle’s speed at the moment of collision with a pedestrian is essential for investigators and law enforcement officers to lawfully and accurately interpret the driver’s actions and contributing factors during a traffic accident. However, only a few objective methods for determining vehicle speed are applicable in expert practice.

This article examines the current state of this issue based on a review of contemporary Russian and international scientific sources. Existing methods are examined based on their core principle. It is shown that objective assessment of a vehicle’s speed at the moment of impact with a stationary obstacle (a pedestrian) is a complex task that requires the use of several approaches with varying degrees of applicability. Further research is needed into the morphological analysis of the conditions and circumstances of traffic accidents involving a moving vehicle and a stationary pedestrian, taking into account the design features of modern vehicles. This will contribute not only to a more thorough and objective investigation of road traffic accidents, but also to the advancement of forensic medicine.

Russian Journal of Forensic Medicine. 2025;11(2):155-165
pages 155-165 views

Case reports

Establishing genetic relationship between a surrogate mother and a child using histological placental blocks: a series of case reports

Konovalenko A.V., Kukharenok I.I.

Abstract

Assisted reproductive technologies are now widely available, and the demand for this type of medical service is growing, necessitating legal control in compliance with the legislation of the Russian Federation.

This publication was prompted by criminal investigations conducted by the Investigative Committee of Russia, which commissioned expert examinations to determine the genetic relationship between a surrogate mother and the child she delivered. The article presents a novel methodology for effectively addressing challenging forensic tasks related to determining a newborn’s biological origin.

Three expert examinations used paraffin-embedded blocks and histological sections of placental tissue, along with reference samples of buccal epithelial cells and blood. Microscopic examination of the histological sections was performed to identify placental components.

Molecular genetic techniques were used to identify maternal and fetal DNA in the provided biological samples, as well as for comparative analysis. These included DNA extraction and quantification, real-time polymerase chain reaction, electrophoretic separation of amplification products, and subsequent analysis.

The examinations made it possible to localize and label the maternal and fetal parts of the placenta within the histological blocks. The children’s blood samples and the surrogate mothers’ buccal epithelial cells were genotyped, and genetic profiles of the labeled samples were determined.

In one of the three cases studied, a comparison of the obtained genotypes revealed a discrepancy between the genetic profiles of the child’s blood and the fetal part of the placenta. In the other two cases, the genetic profiles were confirmed to be identical.

This observation underscores the importance of utilizing placental tissue to avoid erroneous conclusions when establishing maternity in cases involving surrogate mothers. This approach allows the experts to confirm that a specific infant was actually born by the surrogate mother in question.

Russian Journal of Forensic Medicine. 2025;11(2):166-175
pages 166-175 views

Reconstruction of Fall-Related Injury Circumstances: A Case Report

Svetlakov A.V., Khalikov A.A., Sotin A.V.

Abstract

It is not always possible to solve the expert task of determining the circumstances of injury based solely on forensic medical examination findings. In certain cases, analyzing the incident using mathematical calculations allows for the reconstruction of the circumstances under which mechanical trauma occurred.

This paper presents a forensic case that demonstrates the effectiveness of biomechanical (mathematical) analysis as part of a comprehensive forensic biomechanical analysis in reconstructing the circumstances of mechanical trauma resulting from a woman’s fall from the 10th floor under uncertain conditions.

The body of a young woman was discovered on the lawn near a residential building. She had fallen from the balcony window of her apartment on the 10th floor, where she lived with her partner. The Investigative Committee of Russia opened a criminal case based on signs of a potential crime. The woman’s partner claimed she jumped out of the window herself; however, the collected intelligence called his account into question. To address the key investigative question—whether any initial velocity had been imparted to the victim’s body prior to the fall—a comprehensive forensic biomechanical analysis was conducted by forensic medical experts and a mathematical engineer. The constructed mathematical model made it possible to determine which part of the body would have received the impact in the case of a spontaneous fall, i.e., without any external mechanical force. A comparison of the actual medical data and the calculated results revealed that the victim’s body had gained additional initial velocity prior to the fall.

This case demonstrates how a computational experiment can be used as a digital tool to help experts choose between several mechanical injury scenarios.

Russian Journal of Forensic Medicine. 2025;11(2):176-184
pages 176-184 views

Forensic medical and toxicological expertise in a case of administrative license revocation: organizational and methodological considerations (a case report)

Kuznetsov S.V., Molin Y.A., Reiniuk V.L., Sinenchenko A.G., Balabanova O.L., Antonova A.M., Batotsyrenov C.B., Demidova E.O., Varioshkin P.N., Pshennikova D.A., Sukhonos N.Y., Lodiagin G.A.

Abstract

The article discusses the controversial aspects of conducting medical examinations for intoxication. This work is based on a case report on determining the legitimacy of administrative license revocation, which involved a forensic medicine and toxicology expert.

A detailed chronology is provided, from the moment the driver was stopped by a traffic police officer and suspended from driving for an alcohol intoxication test, to the ruling by the magistrates’ court, which was later upheld by the court of cassation.

This case study illustrates errors in interpreting the findings of chemical tests for intoxication, which occur when psychoactive substances are incorrectly identified in human biological fluids. This case of γ-butyrolactone detection in a professional car driver highlights the inadmissibility of unreliable methods for chemical compound identification. Moreover, the paper emphasizes insufficient training of clinical laboratory diagnostics physicians, addresses the issue of an overly mechanistic approach to their work, and proposes potential solutions to the identified shortcomings.

Russian Journal of Forensic Medicine. 2025;11(2):185-195
pages 185-195 views

Postmortem soft tissue damage caused by mice: a case report

Lavrukova O.S., Kobzev A.M.

Abstract

Postmortem injuries inflicted by small rodents such as house and field mice are a significant aspect of forensic medical examination. However, systematic data on this subject remains scarce. Available publications primarily focus on injuries caused by rats, whereas those inflicted by mice receive less attention. This article presents a case illustrating the specific features of soft tissue damage resulting from mouse activity.

A man’s body was discovered in a wooden house in a village, bearing a neck injury. A forensic examination revealed a soft tissue defect on the right lateral surface of the neck, along with signs of its postmortem origin from small rodent teeth (house and field mice). Establishing the postmortem origin of the soft tissue defect caused by small rodent teeth, as well as an intact external jugular vein at the base of the defect, helped to rule out the possibility that the injury was inflicted by a sharp object.

Mice have a less developed masticatory apparatus than rats; as a result, the damage they inflict mostly affects soft tissues. They prefer to gnaw on stiffened tissues (e.g., during the winter months when tissues become more rigid), and this damage has distinct morphological characteristics. Moreover, mouse activity can significantly alter the appearance of antemortem injuries. The presence of animal tissues, hairs, and feces within the damaged area provides additional evidence of rodent activity and necessitates further analysis.

Russian Journal of Forensic Medicine. 2025;11(2):196-203
pages 196-203 views

Retraction

Retraction of the article “Identification of markers of ricin-containing object consumption in blood and urine” (doi: 10.17816/fm11244)

Abstract

The editorial board informs readers of the retraction of the previously published article, "Identification of markers of ricin-containing object consumption in blood and urine" (Russian Journal of Forensic Medicine. 2024;10(4):529–538. doi: 10.17816/fm11244). The reason for the retraction is the identified violations of biomedical ethics in the study presented in the article. The authors could not confirm the fact that the study protocol was approved by the ethics committee before the study was conducted. Also, the authors could not confirm that the study participants were only those who voluntarily signed (themselves or by the legal representatives) an informed consent form for participation in the study or for using of their medical data for scientific purposes.

The original article has been modified (labeled as retracted) and is not recommended by the Journal's editorial board to be read or used for further research.

The editors regret the possible consequences of publishing the results of the study.

Russian Journal of Forensic Medicine. 2025;11(2):204-205
pages 204-205 views


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