TISSUE TYPING BY MEANS OF PCR AND CAPILLARY ELECTROPHORESIS: NEW ASPECTS, REALITY AND ISSUES

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Abstract


«When the DNA is not enough» – an issue that becoming popular among forensic biologists [1]. The reasons for that are methods of tissue typing, that have limited accuracy and gradually becoming obsolete. The purpose of this review is to discuss the integration of new mRNA markers to the routine genetic forensic practice. The review discusses possibilities of utilizing the developed mRNA biomarkers for tissue typing as well as the approaches of combining gene expression analyses with standard sample workflow in forensic and criminal laboratories. Based on data obtained by the foreign researches, new mRNA markers are quite compatible with classical protocols, including PCR and capillary electrophoresis, increase the specificity of immunological methods and do not require rejection of their use. In the near future we can expect commercially developed tissue specific and multiplex assays at markets abroad. Although population diversity of genetic expression does not allow to create a universal multiplex panel, nevertheless the existing tissue mRNA panel can be enriched by the national features.


About the authors

A. S. Bavykin

ООО «Ниармедик плюс»

Author for correspondence.
Email: andrey.bavykin@nearmedic.ru

Russian Federation к.м.н., LLC «Nearmedic Plus› 125252, г. Москва

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