PROSPECTS FOR THE USE OF THE PARAMETERS OF OXIDATIVE MODIFICATION OF SERUM PROTEINS FOR ASSESSMENT OF THE AGONAL PERIOD DURATION

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Abstract


In the article we present results of the studies in cases of death with a long agonal period to determine the length of the agony.

Objectives. The task was to examine the features of oxidative modification of serum proteins in cases of deaths with a long agonal period.

Material and methods. In all experiments we analyzed the liquid blood sampled from 54 corpses of persons of both sexes who died from the violent and natural death in age of 13 to 89 years. The assessment of the oxidative modification of proteins (OMP) was performed by the level of carbonyl derivatives detected in the reaction with 2,4-dinitrophenylhydrazine. The levels of absolute and relative (in terms of protein) total OMP in cases of long agonal period were compared with the counterpart levels in cases of the short agony. The duration of the agonу was determined using information about the circumstances of the case and investigative data with the permission of the authority ordered the forensic examination. The autopsy data also were taken into account.

Results. We revealed the dependence of the OMP level on the duration of the agonal period. The total OMP (in terms of protein) in cases of long agony have decreased by 45.46 %. The data obtained have showed that the parameters of the OMP, taking into account all the relevant factors, can be used to develop criteria for establishing the duration of the agonal period as well as to increase the evidence-based significance of medico-legal conclusions.


About the authors

I. S. Edelev

Department of Clinical Forensic Medicine, Privolzhskii Research Medical University

Email: Edelev11lf133@yandex.ru
ORCID iD: 0000-0002-5852-3758

Russian Federation

Ivan S. Edelev, Privolzhskii Research Medical University

L. M. Obukhova

Department of Biochemistry, Privolzhskii Research Medical University

Email: ObuhovaLM@yandex.ru

Russian Federation

Larisa M. Obukhova, Dr. Sci. (Biol.), Ass. Prof., Privolzhskii Research Medical University

N. A. Andriyanova

Nizhnii Novgorod Regional Bureau of Forensic Medicine

Email: andriynova.chim@yandex.ru

Russian Federation

[Natalya A. Andriyanova, Cand. Sci. (Chem.), Nizhnii Novgorod Regional Bureau of Forensic Medicine

N. S. Edelev

Nizhnii Novgorod Regional Bureau of Forensic Medicine; Department of Clinical Forensic Medicine, Privolzhskii Research Medical University

Author for correspondence.
Email: sudmedex-nn@mail.ru
ORCID iD: 0000-0002-7341-8833

Russian Federation

Nikolai S. Edelev, Dr. Sci. (Med.), Prof., Nizhnii Novgorod Regional Bureau of Forensic Medicine

References

  1. Mazeikiene S., Laima S., Chmieliauskas S., Fomin D., Andriuskeviciute G., Markeviciute M., Matuseviciute А., Yasulaitis А., Stasiuniene J. Deontological examination: Clinical and forensic medical diagnoses discrepancies. Egyptian Journal of Forensic Sciences. 2016;6(4):323-327. https://doi.org/10.1016/j.ejfs.2016.02.003
  2. Rutty G. N., Duerden R. M., Carter N., Clark J. C. Are coroners’ necropsies necessary? A prospective study examining whether a “view and grant” system of death certification could be introduced into England and Wales. J Clin Pathol. 2001;54(4):279-284. http://doi.org/10.1136/jcp.54.4.279
  3. Обухова Л. М., Эделев Н. С., Андриянова Н. А., Эделев И. С. Определение содержания миоглобина в крови в судебно-медицинской практике: методические особенности и перспективы. Судебно-медицинская экспертиза. 2016;(4):57-60. [Obukhova L. M., Edelev N. S., Andriyanova N. A., Edelev I. S. Determination of the blood myoglobin levels for the purpose of forensic medical expertise: The methodological peculiarities and the prospects for the further use. Sudebno-meditsinskaya ekspertiza. 2016;(4):57-60. (In Russ.)] http://doi.org/10.17116/sudmed201659457-60
  4. Эделев Н. С., Андриянова Н. А., Эделев И. С. Изучение влияния причины смерти, давности забора и температуры хранения трупной крови на концентрацию миоглобина. Медицинская экспертиза и право. 2016;(6):47-50. [Edelev N. S., Andri yanova N. A., Edelev I. S. Izuchenie vliyaniya prichiny smerti, davnosti zabora i temperatury khraneniya trupnoi krovi na kontsentratsiyu mioglobina. Meditsinskaya ekspertiza i pravo. 2016;(6):47-50. (In Russ.)]
  5. Эделев Н. С., Эделев И. С. Посмертные изменения соотношения уровня содержания веществ низкой и средней молекулярной массы в сыворотке крови и моче. Медицинская экспертиза и право. 2017;(5):49-51. [Edelev N. S., Edelev I. S. Posthumous changes of the ratio of level of content of substances of low and medium molecular weight in serum of blood and urine. Meditsinskaya ekspertiza i pravo. 2017;(5):49-51. (In Russ.)]
  6. Naito Y., Lee M.-C., Kato Y., Nagai R., Yoshikazu Y. Oxidative stress markers. Anti-aging Medicine. 2010; 7:36-44. https://doi.org/10.3793/jaam.7.36
  7. Пашинян Г. А., Прутовых В. В. Использование метода хемилюминесценции для определения прижизненности и давности механической травмы скелетных мышц. Судебно-медицинская экспертиза. 1978;2:15-17. [Pashinian G. A., Prutovykh V. V. Ispolzovanie metoda khemilyuminestsentsii dlya opredeleniya prizhiznennosti i davnosti mekhanicheskoi travmy skeletnykh myshts. Sudebno-meditsinskaya ekspertiza. 1978;2:15-17. (In Russ.)]
  8. Путинцев В. А., Богомолов Д. В., Богомолова И. Н., Денисова О. П. Определение длительности и темпа умирания по морфологическим признакам. Методические рекомендации. Москва: ФГБУ РЦСМЭ; 2017. [Putintsev V. A., Bogomolov D. V., Bogomolova I. N., Denisova O. P. Opredelenie dlitelnosti i tempa umiraniya po morfologicheskim priznakam. Metodicheskie rekomendatsii. Moscow: Russian Center of Forensic Medical Expertise; 2017. (In Russ.)]
  9. Дубинина Е. Е., Бурмистров С. О., Ходов Д. А., Поротов И. Г. Окислительная модификация белков сыворотки крови человека, метод ее определения. Вопросы медицинской химии. 1995;41(1):24-26. [Dubinina E. E., Burmistrov S. O., Khodov D. A., Porotov I. G. Okislitelnaya modifikatsiya belkov syvorotki krovi cheloveka, metod ee opredeleniya. Voprosy meditsinskoi khimii. 1995;41(1):24-26. (In Russ.)]
  10. Gayen J. R., Zhang K., RamachandraRao S. P., Mahata M., Chen Y., Kim H.-S., Naviaux R. K., Sharma K., Mahata S.K., O’Connor D.T. Role of reactive oxygen species in hyper-adrenergic hypertension: Biochemical, physiological, and pharmacological evidence from targeted ablation of the chromogranin A (Chga) gene. Circulation: Cardiovascular Genetics. 2010;3(5):414-425. https://doi.org/10.1161/CIRCGENETICS.109.924050
  11. Карли Ф. Метаболический ответ на острый стресс. В кн.: Актуальные проблемы анестезиологии- реаниматологии (под ред. Е. В. Недашковского). Архангельск–Тромсе; 1997;31-34. [Karli F. Metabolicheskii otvet na ostryi stress. In: Aktualnye problemy anesteziologii-reanimatologii (E. V. Nedashkovskii, ed.). Arkhangelsk–Tromse; 1997;31-34. (In Russ.)]

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